*Target (protein/gene name): *NCBI Gene # or RefSeq#: *Protein ID (NP or XP #) or Wolbachia#: *Organism (including strain): Etiologic Risk Group (see link below): *Background/Disease Information (sort of like the Intro to your Mini Research Write up):Essentiality of this protein:Complex of proteins?:Druggable Target: *EC#:Link to BRENDA EC# page:--Show screenshot of BRENDA enzyme mechanism schematic Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):-- link to Sigma (or other company) page for assay or assay reagents (substrates)-- link (or citation) to paper that contains assay information-- List cost and quantity of substrate reagents and supplierStructure Available (PDB or Homology model) -- PDB # or closest PDB entry if using homology model: -- For Homology Model option: ---- Show pairwise alignment of your BLASTP search in NCBI against the PDB ---- Query Coverage: ---- Max % Identities: ---- % Positives ---- Chain used for homology: Current Inhibitors:Expression Information (has it been expressed in bacterial cells):Purification Method:Image of protein (PyMol with features delineated and shown separately): *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): *length of your protein in Amino AcidsMolecular Weight of your protein in kiloDaltons using the Expasy ProtParam website Molar Extinction coefficient of your protein at 280 nm wavelength:TMpred graph Image (http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it. *CDS Gene Sequence (paste as text only): *GC% Content for gene: *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):*GC% Content for gene (codon optimized): Do Not Need this info for Spring (but still copy these lines to your Target page for now)Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):(link to DNA Works output text file - that should be saved in your Google Docs folder after you did the primer design protocol) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to. Primer design results for 'tail' primers (this is just 2 sequences):
*NCBI Gene # or RefSeq#:
*Protein ID (NP or XP #) or Wolbachia#:
*Organism (including strain):
Etiologic Risk Group (see link below):
*Background/Disease Information (sort of like the Intro to your Mini Research Write up): Essentiality of this protein: Complex of proteins?: Druggable Target:
*EC#: Link to BRENDA EC# page: -- Show screenshot of BRENDA enzyme mechanism schematic
Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): -- link to Sigma (or other company) page for assay or assay reagents (substrates) -- link (or citation) to paper that contains assay information -- List cost and quantity of substrate reagents and supplier Structure Available (PDB or Homology model) -- PDB # or closest PDB entry if using homology model: -- For Homology Model option: ---- Show pairwise alignment of your BLASTP search in NCBI against the PDB ---- Query Coverage: ---- Max % Identities: ---- % Positives ---- Chain used for homology:
Current Inhibitors: Expression Information (has it been expressed in bacterial cells): Purification Method: Image of protein (PyMol with features delineated and shown separately):
*Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):
*length of your protein in Amino Acids Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website
Molar Extinction coefficient of your protein at 280 nm wavelength: TMpred graph Image (http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
*CDS Gene Sequence (paste as text only):
*GC% Content for gene:
*CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): *GC% Content for gene (codon optimized):
Do Not Need this info for Spring (but still copy these lines to your Target page for now) Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): (link to DNA Works output text file - that should be saved in your Google Docs folder after you did the primer design protocol) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
Primer design results for 'tail' primers (this is just 2 sequences):
Resources:
See ProtocolTargetDiscoveryVDS.docx for more Etiologic Risk Group Categories (for pathogens): http://www.utexas.edu/research/rsc/ibc/agent_class.html#_Toc7238334Databases of genes/organisms: http://www.niaid.nih.gov/Pages/default.aspx http://eupathdb.org/eupathdb/ https://patricbrc.vbi.vt.edu/portal/portal/patric/Home http://www.nmpdr.org/FIG/wiki/view.cgi/Main/EssentialGenes http://tubic.tju.edu.cn/deg/ http://csgid.org/csgid/cake/pages/community_request_gateway http://tdrtargets.org/ http://gsc.jcvi.org/status.shtml
Scientific Nomenclature page from Center for Disease Control (gene, protein names and abbreviations) http://wwwnc.cdc.gov/eid/pages/scientific-nomenclature.htm
Gene Information: NCBI GENE Page: http://www.ncbi.nlm.nih.gov/gene BLAST Page: http://blast.ncbi.nlm.nih.gov/
Protein Information: NCBI Protein Page: http://www.ncbi.nlm.nih.gov/protein Protein Expression Website Protein Expression Paper: SGC_ProteinProductionPurificationNatMethods2008.pdf
Primer Overlap PCR Articles HooverLubkowski_PCRoverlapcloninggnf042.pdf StemmerPCRoverlapGene1995.pdf
Is my target good for Virtual Screening programs? Reynolds_THermodynamicsLigandBinding_MedChemLett2011.pdf